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May 20, 2020 在生物科学和相关领域中,培养一个或多个细胞可能是有用的。本发明的一些实施方案包括用于在微流体装置中培养细胞或细胞群的装置和方法。发明内容一方面,提供了用于培养一个或多个生物细胞的微流体装置,其包括:液流区域(flowregion),其被配置为含有第一流体培养基的流;和至少一个生长 This protocol is used to measure sodium currents from heterologously transfected cells lines, such as HEK293 cells. Standard whole cell patch clamp technique is used to assess ion channel function. This protocol has been used to study cardiac type Nav1.5 sodium channel, and in particular to compare wild-type and mutant channels related to Brugada Syndrome (BrS). Cytoplasmic localization of mRNAs is common to all organisms and serves the spatial expression of genes. Cis-acting RNA signals (mostly found in the mRNA’s 3'-UTR), called zipcodes recruit trans-acting RNA-binding proteins that facilitate the localization of the mRNA. UV-cross-linking or affinity purification has been applied to identify such proteins but suffer from the need for stable RNA
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